Recombinant Human HDAC3 Protein, His-tagged HDAC3-46H

Recombinant Human HDAC3 Protein, His-tagged

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Recombinant Human HDAC3 Protein, His-tagged

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Cat.No. : HDAC3-46H
Product Overview : Recombinant human HDAC3 protein with a C-terminal His-tag and human NCOR2(395-489) protein with a N-terminal GST-tag were expressed in baculovirus expression vector system.
Source : Insect cells
Species : Human
Tag : His
Molecular Mass : 49.7 kDa
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC substrate 3
Stability : ≥ 6 months
Storage : Store it at -80 centigrade
Storage Buffer : 40 mM Tris-HCl pH 8.0, 110 mM sodium chloride, 2.2 mM potassium chloride, 250 mM imidazole, and 20% glycerol
SDS-PAGE Analysis of HDAC3/NCOR2 :

Lane 1: MW Markers; Lane 2: HDAC3/NCOR2 (4 μg) Complex of human HDAC3: C-terminal His-tag HDAC3, MW=50 kDa, and human NCOR2 with N-terminal GST tag, MW=38 kDa. Representative gel image shown; actual purity may vary between each batch.

HDAC3/NCOR2 Deacetylase Activity :

Gene Name : HDAC3 histone deacetylase 3 [ Homo sapiens (human) ]
Official Symbol : HDAC3
Synonyms : HDAC3; histone deacetylase 3; HD3; RPD3; KDAC3; RPD3-2; histone deacetylase 3; histone deacetylase 3
Gene ID : 8841
mRNA Refseq : NM_003883
Protein Refseq : NP_003874
MIM : 605166
UniProt ID : O15379

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