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Active Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated

Cat.No. : IL23A&IL12B-334H
Product Overview : Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # NP_057668 (IL23A) & AAH67499 (IL12B)). Predicted N-terminus: His (IL23A) & Ile 23 (IL12B). Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag, followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Anti-p40 subunit of IL23(Anti-IL12B) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 4.9-39 ng/mL (Routinely tested).
Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 1.2-19.5 ng/mL (QC tested).
Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173ng/ml (Routinely tested).
Cell based assay shows that the secretion of IL-17 induced by Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.25-1.40 ng/mL (Routinely tested).
Molecular Mass : 21.9 kDa (IL23A) & 34.7 kDa (IL12B)
Protein length : Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B)
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ] & IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
Official Symbol : IL23A & IL12B
Synonyms : IL23A; IL 23; IL 23A; IL23P19; P19; SGRF; IL-23-A; IL-23p19; IL-23; IL-23A; MGC79388; & IL12B; NKSF2; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; NKSF; CLMF p40; IL-12B;
Gene ID : 51561 & 3593
mRNA Refseq : NM_016584 & NM_002187
Protein Refseq : NP_057668 & NP_002178
MIM : 605580 & 161561
UniProt ID : Q9NPF7 & P29460

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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