Recombinant Mouse IL23A&IL12B protein, His-tagged

• Cat.No.: IL23A&IL12B-1614M
• Product Overview: Recombinant Mouse IL23A&IL12B protein(Q9EQ14-1(IL-23 alpha)&P43432-1(IL-12 beta))(Val22-Ala196(IL23A) & Met23-Ser335(IL12B)), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Val22-Ala196(IL23A) & Met23-Ser335(IL12B)
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 16160

Gene Information

• Gene Name: Il23a interleukin 23, alpha subunit p19 [ Mus musculus ]
• Official Symbol: IL23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19; p19; IL-23
• Gene ID: 83430
• mRNA Refseq: NM_031252
• Protein Refseq: NP_112542
• Gene Name 2: Il12b interleukin 12b [ Mus musculus ]
• Official Symbol 2: IL12B
• Synonyms 2: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• mRNA Refseq 2: NM_008352
• Protein Refseq 2: NP_032378