Active Recombinant Human LAG3 Protein, His & Avi-tagged, Biotinylated
Cat.No. : | LAG3-384H |
Product Overview : | Recombinant Human LAG3 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1). Predicted N-terminus: Leu 23. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 49.9 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
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Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Anti-Human LAG-3 Mab at 2 μg/mL (100 μL/well) can bind Recombinant Human LAG3 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.5-16 ng/mL (QC tested). |
Molecular Mass : | 49.9 kDa |
Protein length : | Leu 23 - Leu 450 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | LAG3 lymphocyte-activation gene 3 [ Homo sapiens ] |
Official Symbol : | LAG3 |
Synonyms : | LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223; |
Gene ID : | 3902 |
mRNA Refseq : | NM_002286 |
Protein Refseq : | NP_002277 |
MIM : | 153337 |
UniProt ID : | P18627 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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