Recombinant Human lipoic acid synthetase Protein, His&Flag&StrepII tagged

• Cat.No.: LIAS-002H
• Product Overview: Recombinant human LIAS protein (O43766) (Leu 28-Leu 372) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 28-372aa
• Description: The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. Localized in the mitochondrion, this iron-sulfur enzyme catalyzes the final step in the de novo pathway for the biosynthesis of lipoic acid, a potent antioxidant. The deficient expression of this enzyme has been linked to conditions such as diabetes, atherosclerosis and neonatal-onset epilepsy. Alternative splicing occurs at this locus, and several transcript variants encoding distinct isoforms have been identified. Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 45.6 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: LIAS lipoic acid synthetase [ Homo sapiens (human) ]
• Official Symbol: LIAS
• Synonyms: LIAS; lipoic acid synthetase; lipoyl synthase, mitochondrial; LAS; lip-syn; lipoate synthase; LS; LIP1; PDHLD; HUSSY-01; MGC23245
• Gene ID: 11019
• mRNA Refseq: NM_006859
• Protein Refseq: NP_006850
• MIM: 607031
• UniProt ID: O43766