Recombinant Human MGEA5 Protein, GST-tagged

• Cat.No.: MGEA5-5301H
• Product Overview: Human MGEA5 full-length ORF ( NP_036347.1, 1 a.a. - 916 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 129.3 kDa
• AA Sequence: MVQKESQATLEERESELSSNPAASA GASLEPPAAPAPGEDNPAGAGGAAV AGAAGGARRFLCGVVEGFYGRPWVM EQRKELFRRLQKWELNTYLYAPKDD YKHRMFWREMYSVEEAEQLMTLISA AREYEIEFIYAISPGLDITFSNPKE VSTLKRKLDQVSQFGCRSFALLFDD IDHNMCAADKEVFSSFAHAQVSITN EIYQYLGEPETFLFCPTEYCGTFCY PNVSQSPYLRTVGEKLLPGIEVLWT GPKVVSKEIPVESIEEVSKIIKRAP VIWDNIHANDYDQKRLFLGPYKGRS TELIPRLKGVLTNPNCEFEANYVAI HTLATWYKSNMNGVRKDVVMTDSED STVSIQIKLENEGSDEDIETDVLYS PQMALKLALTEWLQEFGVPHQYSSR QVAHSGAKASVVDGTPLVAAPSLNA TTVVTTVYQEPIMSQGAALSGEPTT LTKEEEKKQPDEEPMDMVVEKQEET DHKNDNQILSEIVEAKMAEELKPMD TDKESIAESKSPEMSMQEDCISDIA PMQTDEQTNKEQFVPGPNEKPLYTA EPVTLEDLQLLADLFYLPYEHGPKG AQMLREFQWLRANSSVVSVNCKGKD SEKIEEWRSRAAKFEEMCGLVMGMF TRLSNCANRTILYDMYSYVWDIKSI MSMVKSFVQWLGCRSHSSAQFLIGD QEPWAFRGGLAGEFQRLLPIDGAND LFFQPPPLTPTSKVYTIRPYFPKDE ASVYKICREMYDDGVGLPFQSQPDL IGDKLVGGLLSLSLDYCFVLEDEDG ICGYALGTVDVTPFIKKCKISWIPF MQEKYTKPNGDKELSEAEKIMLSFH EEQEVLPETFLANFPSLIKMDIHKK VTDPSVAKSMMACLLSSLKANGSRG AFCEVRPDDKRILEFYSKLGCFEIA KMEGFPKDVVILGRSL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MGEA5 meningioma expressed antigen 5 (hyaluronidase) [ Homo sapiens ]
• Official Symbol: MGEA5
• Synonyms: MGEA5; meningioma expressed antigen 5 (hyaluronidase); bifunctional protein NCOAT; MEA5; NCOAT; nuclear cytoplasmic O GlcNAcase and acetyltransferase; OGA; O-GlcNAcase; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; FLJ11229; FLJ23355; KIAA0679
• Gene ID: 10724
• mRNA Refseq: NM_001142434
• Protein Refseq: NP_001135906
• MIM: 604039
• UniProt ID: O60502