Recombinant Human MME Protein, His-tagged

• Cat.No.: MME-1113H
• Product Overview: Recombinant Human MME Protein (Tyr52-Trp750) with His tag was expressed in 293F.

Specification

• Description: The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
• Source: HEK293F
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 81.6 kDa; Accurate Molecular Mass: 100 kDa
• Protein length: Tyr52-Trp750
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MME membrane metalloendopeptidase [ Homo sapiens (human) ]
• Official Symbol: MME
• Synonyms: MME; membrane metalloendopeptidase; NEP; SFE; CD10; CALLA; CMT2T; SCA43; neprilysin; atriopeptidase; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); neprilysin-390; neprilysin-411; neutral endopeptidase 24.11; skin fibroblast elastase; EC 3.4.24.11
• Gene ID: 4311
• mRNA Refseq: NM_000902
• Protein Refseq: NP_000893
• MIM: 120520
• UniProt ID: P08473