| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
N/A |
| Form : |
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Fluorescent Human CD20 Full Length Protein-VLP at 5 μg/mL (100 μL/well) can bind Rituximab with a linear range of 5-39 ng/mL.Immobilized Fluorescent Human CD20 Full Length Protein-VLP at 5 μg/mL (100 μL/well) can bind Ofatumumab with a linear range of 1-20 ng/mL. |
| Molecular Mass : |
This protein carries a GFP tagVirus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening. The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display. |
| Protein Length : |
Met 1 - Pro 297 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
