Active Recombinant Human MSLN Protein (Glu 296 - Gly 580), His-tagged, GFP Fusion
Cat.No. : | MSLN-317H |
Product Overview : | Recombinant Human MSLN Protein, His-tagged, GFP Fusion is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1). Predicted N-terminus: Ala 2. This protein carries a GFP tag at the N-terminus and a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.4 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (GFP): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 530 nm. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind Recombinant Human MSLN Protein, His-tagged, GFP Fusion with a linear range of 0.039-0.625 μg/mL (QC tested). Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind Recombinant Human MSLN Protein, His-tagged, GFP Fusion with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200) (Routinely tested). 2e5 of the MSLN-CAR-293 cells were stained with 100ul of 3 μg/mL of Recombinant Human MSLN Protein, His-tagged, GFP Fusion. GFP was used as negative control (Routinely tested). |
Molecular Mass : | 60.4 kDa |
Protein length : | Glu 296 - Gly 580 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Applications : | Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | MSLN mesothelin [ Homo sapiens ] |
Official Symbol : | MSLN |
Synonyms : | MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; SMRP; |
Gene ID : | 10232 |
mRNA Refseq : | NM_001177355 |
Protein Refseq : | NP_001170826 |
MIM : | 601051 |
UniProt ID : | Q13421 |
Products Types
◆ Recombinant Protein | ||
MSLN-001H | Active Recombinant Human MSLN Protein, His-tagged | +Inquiry |
MSLN-16H | Active Recombinant Human MSLN Protein, His-tagged, Alexa Fluor® 647 conjugated | +Inquiry |
MSLN-8392H | Active Recombinant Human MSLN Protein | +Inquiry |
MSLN-318H | Recombinant Human MSLN Protein (Glu 296 - Gly 580), His-tagged, PE-Labeled | +Inquiry |
MSLN-1139H | Recombinant Human MSLN Protein, His-tagged | +Inquiry |
◆ Lysates | ||
MSLN-1343HCL | Recombinant Human MSLN cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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