Recombinant Human OGT Protein (606-1022 aa), His-SUMO-tagged
Cat.No. : | OGT-700H |
Product Overview : | Recombinant Human OGT Protein (606-1022 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Neuroscience. Protein Description: Partial. |
- Specification
- Gene Information
- Related Products
Description : | Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in Cytoplasmic domain and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity . Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) . As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues . O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex . Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. |
Source : | E. coli |
Species : | Human |
Tag : | His-SUMO |
Form : | Tris-based buffer, 50% glycerol |
Molecular Mass : | 62.5 kDa |
Protein length : | 606-1022 aa |
AA Sequence : | MAEANHFIDLSQIPCNGKAADRIHQ DGIHILVNMNGYTKGARNELFALRP APIQAMWLGYPGTSGALFMDYIITD QETSPAEVAEQYSEKLAYMPHTFFI GDHANMFPHLKKKAVIDFKSNGHIY DNRIVLNGIDLKAFLDSLPDVKIVK MKCPDGGDNADSSNTALNMPVIPMN TIAEAVIEMINRGQIQITINGFSIS NGLATTQINNKAATGEEVPRTIIVT TRSQYGLPEDAIVYCNFNQLYKIDP STLQMWANILKRVPNSVLWLLRFPA VGEPNIQQYAQNMGLPQNRIIFSPV APKEEHVRRGQLADVCLDTPLCNGH TTGMDVLWAGTPMVTMPGETLASRV AASQLTCLGCLELIAKNRQEYEDIA VKLGTDLEYLKKVRGKVWKQRISSP LFNTKQYTMELERLYLQ |
Purity : | > 90% as determined by SDS-PAGE. |
Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
Concentration : | A hardcopy of COA with concentration instruction is sent along with the products. |
Gene Name : | OGT O-linked N-acetylglucosamine (GlcNAc) transferase [ Homo sapiens (human) ] |
Official Symbol : | OGT |
Synonyms : | OGT; HRNT1; O-GLCNAC; EC 2.4.1.255 |
Gene ID : | 8473 |
mRNA Refseq : | NM_181672 |
Protein Refseq : | NP_858058 |
MIM : | 300255 |
UniProt ID : | O15294 |
Products Types
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Ogt-369M | Recombinant Mouse Ogt Protein, MYC/DDK-tagged | +Inquiry |
OGT-6327M | Recombinant Mouse OGT Protein, His (Fc)-Avi-tagged | +Inquiry |
OGT-1576H | Recombinant Human OGT Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
OGT-3589HCL | Recombinant Human OGT 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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