Recombinant Human PPBP Protein

• Cat.No.: PPBP-049H
• Product Overview: Recombinant human Platelet-derived growth factor AB (rhPDGF-AB) contain P04085 (alpha chain) over Ser87–Thr211 aa positions of the precursor and P01127 (beta chain) over aa Ser82–Ile191 positions of the precursor, which was expressed in human 293 cells (H

Specification

• Description: The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. The protein also is an antimicrobial protein with bactericidal and antifungal activity. [provided by RefSeq, Nov 2014]
• Source: HEK293 cells
• Species: Human
• Molecular Mass: rhPDGF-AB is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of a 14.3 kDa (alpha-chain) and 12.2 kDa (beta-chain), having a total molecular mass of 26.5 kDa. In DTT-reduced SDS-PAGE, rhPDGF-AB protein migrates as 17.5 kDa (alpha-chain) and 12.5 kDa (beta-chain) poly peptide.
• Endotoxin: Less than 0.01 ng endotoxin/ 1 µg rhPDGF-AB.
• Purity: >90% as determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) rhPDGF-AB. All lots are greater than 90% pure.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhPDGF-AB should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: PPBP pro-platelet basic protein (chemokine (C-X-C motif) ligand 7) [ Homo sapiens ]
• Official Symbol: PPBP
• Synonyms: PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); THBGB1; platelet basic protein; b TG1; Beta TG; beta thromboglobulin; connective tissue activating peptide III; CTAP3; CTAPIII; CXCL7; LA PF4; LDGF; MDGF; NAP 2; NAP 2 L1; neutrophil activating peptide 2; PBP; SCYB7; TGB; TGB1; thrombocidin 1; thrombocidin 2; beta-thromboglobulin; CXC chemokine ligand 7; C-X-C motif chemokine 7; thromboglobulin, beta-1; small inducible cytokine B7; small-inducible cytokine B7; leukocyte-derived growth factor; low-affinity platelet factor IV; neutrophil-activating peptide 2; neutrophil-activating peptide-2; macrophage-derived growth factor; connective tissue-activating peptide III; small inducible cytokine subfamily B, member 7; TC1; TC2; B-TG1; NAP-2; THBGB; LA-PF4; SCAR10; Beta-TG; CTAP-III
• Gene ID: 5473
• mRNA Refseq: NM_002704
• Protein Refseq: NP_002695
• MIM: 121010
• UniProt ID: P02775