Recombinant Human RRAGA Protein (1-247 aa), GST-tagged RRAGA-1197H

Recombinant Human RRAGA Protein (1-247 aa), GST-tagged

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Recombinant Human RRAGA Protein (1-247 aa), GST-tagged

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Cat.No. : RRAGA-1197H
Product Overview : Recombinant Human RRAGA Protein (1-247 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Apoptosis. Protein Description: Partial.
Description : Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Source : E. coli
Species : Human
Tag : GST
Form : Tris-based buffer,50% glycerol
Molecular Mass : 56.1 kDa
Protein length : 1-247 aa
AA Sequence : MPNTAMKKKVLLMGK SGSGKTSMRSIIFAN YIARDTRRLGATIDV EHSHVRFLGNLVLNL WDCGGQDTFMENYFT SQRDNIFRNVEVLIY VFDVESRELEKDMHY YQSCLEAILQNSPDA KIFCLVHKMDLVQED QRDLIFKEREEDLRR LSRPLECACFRTSIW DETLYKAWSSIVYQL IPNVQQLEMNLRNFA QIIEADEVLLFERAT FLVISHYQCKEQRDV HRFEKISNIIKQFKL SCSKLAA
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : RRAGA Ras-related GTP binding A [ Homo sapiens ]
Official Symbol : RRAGA
Synonyms : FIP1; RAGA; FIP-1;
Gene ID : 10670
mRNA Refseq : NM_006570.4
Protein Refseq : NP_006561.1
MIM : 612194
UniProt ID : Q7L523

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