Recombinant Human RRAGA Protein (1-247 aa), GST-tagged

• Cat.No.: RRAGA-1197H
• Product Overview: Recombinant Human RRAGA Protein (1-247 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Apoptosis. Protein Description: Partial.

Specification

• Description: Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 56.1 kDa
• Protein length: 1-247 aa
• AA Sequence: MPNTAMKKKVLLMGKSGSGKTSMRS IIFANYIARDTRRLGATIDVEHSHV RFLGNLVLNLWDCGGQDTFMENYFT SQRDNIFRNVEVLIYVFDVESRELE KDMHYYQSCLEAILQNSPDAKIFCL VHKMDLVQEDQRDLIFKEREEDLRR LSRPLECACFRTSIWDETLYKAWSS IVYQLIPNVQQLEMNLRNFAQIIEA DEVLLFERATFLVISHYQCKEQRDV HRFEKISNIIKQFKLSCSKLAA
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: RRAGA Ras-related GTP binding A [ Homo sapiens ]
• Official Symbol: RRAGA
• Synonyms: FIP1; RAGA; FIP-1
• Gene ID: 10670
• mRNA Refseq: NM_006570.4
• Protein Refseq: NP_006561.1
• MIM: 612194
• UniProt ID: Q7L523