Recombinant Human SIRPA protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: SIRPA-324HB
• Product Overview: Biotinylated Recombinant Human Sirpa Protein is expressed from Expi293 with a Fc and Avi Tag at the C-terminal. It contains Glu31-Arg370.

Specification

• Description: Signal regulatory protein α(SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons. SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. Cancer cells highly expressed CD47 that activate SIRPα and inhibit macrophagemediated destruction.
• Source: HEK293
• Species: Human
• Tag: Fc-Avi
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result.
• Protein length: Glu31-Arg370
• Endotoxin: Less than 0.05 EU per μg by the LAL method.
• Purity: >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.

Gene Information

• Gene Name: SIRPA
• Official Symbol: SIRPA
• Synonyms: CD172 antigen-like family member A; CD172a antigen; CD172a;BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1
• Gene ID: 140885
• mRNA Refseq: NM_001040022.1
• Protein Refseq: NP_001035111.1
• MIM: 602461
• UniProt ID: P78324