|Product Overview:||Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). Predicted N-terminus: Glu 31. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.9 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.|
|Tag :||His & Avi|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human CD47, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated with a linear range of 0.078-0.625 μg/mL (QC tested).|
|Molecular Mass:||39.9 kDa|
|Protein length:||Glu 31 - Arg 370|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||SIRPA signal-regulatory protein alpha [ Homo sapiens ]|
|Synonyms:||SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; MYD-1; CD172A; PTPNS1;|