Active Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated
Cat.No. : | SIRPA-347H |
Product Overview : | Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). Predicted N-terminus: Glu 31. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.9 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
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Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human CD47, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated with a linear range of 0.078-0.625 μg/mL (QC tested). |
Molecular Mass : | 39.9 kDa |
Protein length : | Glu 31 - Arg 370 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
Official Symbol : | SIRPA |
Synonyms : | SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; MYD-1; CD172A; PTPNS1; |
Gene ID : | 140885 |
mRNA Refseq : | NM_001040022 |
Protein Refseq : | NP_001035111 |
MIM : | 602461 |
UniProt ID : | P78324 |
Products Types
◆ Lysates | ||
SIRPA-2298HCL | Recombinant Human SIRPA cell lysate | +Inquiry |
SIRPA-1650MCL | Recombinant Mouse SIRPA cell lysate | +Inquiry |
SIRPA-1005RCL | Recombinant Rat SIRPA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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