"SLAMF6" Related Products

Expand

Recombinant Human SLAMF6 protein, His-tagged

Cat.No.: SLAMF6-241H
Product Overview: Recombinant Human SLAMF6(Gln22-Met226) fused with His tag at C-terminal was expressed in HEK293.
Description: NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Mature human NTB-A consists of a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs). An alternatively spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%-34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils. It interacts homophilically through weak associations between the Ig-V domains. NTB-A functions as an activating coreceptor on NK and T cells. Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells. Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells. This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM. NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators. In B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity. In addition, NTB-A binds to the influenza virus hemagglutinin (HA) protein which co-stimulates NK cell activation. The Vpu protein encoded by HIV-1 interferes with NTB-A glycosylation and cell surface expression.
Source: HEK293
Species: Human
Tag : His
Predicted N Terminal: No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human NTB-A/SLAMF6 is coated at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human NTB-A/SLAMF6 Fc Chimera that produces 50% optimal binding response is 0.6-3 μg/mL.
Molecular Mass: Predicted Molecular Mass: 24 kDa
SDS-PAGE: 34-42 kDa, reducing conditions
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
6 months, -70 centigrade under sterile conditions after opening.
Reconstitution: Reconstitute at 500 μg/mL in PBS.
Gene Name: SLAMF6 SLAM family member 6 [ Homo sapiens ]
Official Symbol: SLAMF6
Synonyms: SLAMF6; SLAM family member 6; CD352; KALI; KALIb; Ly108; NTB A; NTBA; SF2000; NTBA receptor; NK-T-B-antigen; activating NK receptor; natural killer-, T- and B-cell antigen; NTB-A; FLJ50657; MGC104953;
Gene ID: 114836
mRNA Refseq: NM_001184714
Protein Refseq: NP_001171643
MIM: 606446
UniProt ID: Q96DU3

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.