Recombinant Human SPHK2 Protein, His-tagged

• Cat.No.: SPHK2-051H
• Product Overview: Active recombinant N-terminal His-tagged full length human SPHK2 protein (2-654) was expressed in insect cells.

Specification

• Description: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Insect cell
• Species: Human
• Tag: His
• Molecular Mass: 72.9 kDa
• Protein length: 2-654
• Purity: ≥75% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 μmol of ADP per minute at 25 centigrade in 20 mM HEPES, pH 7.4, containing 50 mM sodium chloride, 10 mM magnesium chloride, 1mM EGTA, 0.02% triton X-100, and 50 μM sphingosine (d18:1).
• Applications: WB, ELISA, and enzymatic assays
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% Triton X-100, and 5% glycerol

Gene Information

• Gene Name: SPHK2 sphingosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: SPHK2
• Synonyms: SPHK2; sphingosine kinase 2; SK 2; SK-2; SPK 2; SPK-2; sphingosine kinase 2; EC 2.7.1.91
• Gene ID: 56848
• mRNA Refseq: NM_020126
• Protein Refseq: NP_064511
• MIM: 607092
• UniProt ID: Q9NRA0