Active Recombinant Human TIGIT protein, Fc-tagged

• Cat.No.: TIGIT-107H
• Product Overview: Recombinant Human TIGIT protein (Met1-Pro141), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human CD155a-His at 10μg/mL (100μL/well) can bind human TIGIT-Fc, the EC50 of human TIGIT-Fc is 0.03-0.2μg/mL.
• Molecular Mass: The recombinant human TIGIT consists 353 amino acids and predicts a molecular mass of 39.2 kDa. As a result of glycosylation, human TIGIT migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Pro141
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TIGIT T cell immunoreceptor with Ig and ITIM domains [ Homo sapiens ]
• Official Symbol: TIGIT
• Synonyms: TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM
• Gene ID: 201633
• mRNA Refseq: NM_173799
• Protein Refseq: NP_776160
• MIM: 612859
• UniProt ID: Q495A1