Active Recombinant Human TNFRSF1A protein, His-tagged
Cat.No. : | TNFRSF1A-3189H |
Product Overview : | Recombinant Human TNFRSF1A protein(NP_001056.1)(Met1-Thr211), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL.2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.4μg/mL in the presence of 0.25 ng/mL recombinant human TNFα. |
Molecular Mass : | The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Thr211 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 92 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ] |
Official Symbol : | TNFRSF1A |
Synonyms : | TNFRSF1A; tumor necrosis factor receptor superfamily, member 1A; TNFR1; tumor necrosis factor receptor superfamily member 1A; CD120a; TNF R; TNF R I; TNF R55; TNFAR; TNFR60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta; FPF; p55; p60; TBP1; TNF-R; p55-R; TNFR55; TNF-R-I; TNF-R55; MGC19588; |
Gene ID : | 7132 |
mRNA Refseq : | NM_001065 |
Protein Refseq : | NP_001056 |
MIM : | 191190 |
UniProt ID : | P19438 |
Products Types
◆ Recombinant Protein | ||
Tnfrsf1a-314M | Recombinant Mouse TNFRSF1A Protein (ECD), Fc-His-tagged(C-ter) | +Inquiry |
Tnfrsf1a-141M | Recombinant Mouse Tnfrsf1a Protein | +Inquiry |
TNFRSF1A-444H | Recombinant Human TNFRSF1A Protein, His-tagged | +Inquiry |
TNFRSF1A-445H | Recombinant Human TNFRSF1A Protein, His-tagged | +Inquiry |
Tnfrsf1a-10624M | Recombinant Mouse Tnfrsf1a Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
TNFRSF1A-1693MCL | Recombinant Mouse TNFRSF1A cell lysate | +Inquiry |
TNFRSF1A-2390HCL | Recombinant Human TNFRSF1A cell lysate | +Inquiry |
TNFRSF1A-1084RCL | Recombinant Rat TNFRSF1A cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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