Active Recombinant Human TNFSF13B Protein, His & Avi-tagged, Biotinylated

• Cat.No.: TNFSF13B-377H
• Product Overview: Recombinant Human TNFSF13B Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag. The protein has a calculated MW of 29.2 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human BCMA, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFSF13B protein, His & Avi-tagged, Biotinylated with a linear range of 0.24-3.9 ng/mL (QC tested).
  Recombinant Human BCMA, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFSF13B protein, His & Avi-tagged, Biotinylated with a linear range of 0.24-3.9 ng/mL (Routinely tested).
• Molecular Mass: 29.2 kDa
• Protein length: Ala 134 - Leu 285
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; TNFSF20; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; DTL; ZTNF4; TALL-1
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• MIM: 603969
• UniProt ID: Q9Y275