|Product Overview :||Recombinant Marmoset TNFRSF4 protein (Leu29-Ala214), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40/TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4/OX40L/GP34 and can interacts with TRAF2, TRAF3 and TRAF5.|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 21.8 kDa. The protein migrates as 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||186|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of TNFRSF4-340M :||
Marmoset OX40 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of TNFRSF4-340M :||
Immobilized Human OX40 Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Marmoset OX40, His Tag with a linear range of 0.078-1.25 μg/mL (QC tested).
Immobilized Marmoset OX40, His Tag at 2 μg/mL (100 μL/well) can bind Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag with a linear range of 0.2-3 ng/mL (Routinely tested).
|Gene Name :||TNFRSF4|
|Official Symbol :||TNFRSF4|
|Synonyms :||ACT35, CD134, IMD16, OX40, TXGP1L|
|Gene ID :||100408049|
|mRNA Refseq :||XM_002750177.2|
|Protein Refseq :||XP_002750223.1|
|UniProt ID :||F7DHA3|