Active Recombinant Mouse Ace2 Protein, His-tagged

• Cat.No.: Ace2-07M
• Product Overview: Recombinant mouse ACE-2 (18-740 aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A. By cleavage of angiotensin II, may be an important regulator of heart function. By cleavage of angiotensin II, may also have a protective role in acute lung injury. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19, regulating its trafficking on the cell surface and its activity.
• Source: Insect Cell
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 200 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH per minute at pH 7.5, at 25 centigrade.
• Molecular Mass: 84.5 kDa
• Protein length: 731
• AA Sequence: QSLTEENAKTFLNNFNQEAEDLSYQ SSLASWNYNTNITEENAQKMSEAAA KWSAFYEEQSKTAQSFSLQEIQTPI IKRQLQALQQSGSSALSADKNKQLN TILNTMSTIYSTGKVCNPKNPQECL LLEPGLDEIMATSTDYNSRLWAWEG WRAEVGKQLRPLYEEYVVLKNEMAR ANNYNDYGDYWRGDYEAEGADGYNY NRNQLIEDVERTFAEIKPLYEHLHA YVRRKLMDTYPSYISPTGCLPAHLL GDMWGRFWTNLYPLTVPFAQKPNID VTDAMMNQGWDAERIFQEAEKFFVS VGLPHMTQGFWANSMLTEPADGRKV VCHPTAWDLGHGDFRIKMCTKVTMD NFLTAHHEMGHIQYDMAYARQPFLL RNGANEGFHEAVGEIMSLSAATPKH LKSIGLLPSDFQEDSETEINFLLKQ ALTIVGTLPFTYMLEKWRWMVFRGE IPKEQWMKKWWEMKREIVGVVEPLP HDETYCDPASLFHVSNDYSFIRYYT RTIYQFQFQEALCQAAKYNGSLHKC DISNSTEAGQKLLKMLSLGNSEPWT KALENVVGARNMDVKPLLNYFQPLF DWLKEQNRNSFVGWNTEWSPYADQS IKVRISLKSALGANAYEWTNNEMFL FRSSVAYAMRKYFSIIKNQTVPFLE EDVRVSDLKPRVSFYFFVTSPQNVS DVIPRSEVEDAIRMSRGRINDVFGL NDNSLEFLGIHPTLEPPYQPPVT
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Ace2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Mus musculus (house mouse) ]
• Official Symbol: Ace2
• Synonyms: Ace2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; 2010305L05Rik; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; EC 3.4.17.23
• Gene ID: 70008
• mRNA Refseq: NM_027286
• Protein Refseq: NP_081562
• UniProt ID: Q8R0I0