Recombinant Mouse Epo Protein

Cat.No.: Epo-966M
Product Overview: Recombinant mouse Epo (NP_031968.1) (Met1-Arg192) was expressed.
Source: HEK293
Species: Mouse
Predicted N Terminal: Ala 27
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
Molecular Mass: The recombinant mouse Epo consists 166 amino acids and predicts a molecular mass of 18.6 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Epo erythropoietin [ Mus musculus ]
Official Symbol: Epo
Synonyms: EPO; erythropoietin;
Gene ID: 13856
mRNA Refseq: NM_007942
Protein Refseq: NP_031968
MIM:
UniProt ID:
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic c
Function: erythropoietin receptor binding; eukaryotic cell surface binding; hormone activity; protein kinase activator activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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