| Description : |
Interleukin-11 (IL-11) is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell. |
| Source : |
HEK 293 |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 3 ng/mL, measured in a cell proliferation assay using T11 cells. |
| Molecular Mass : |
~21 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
GPPAGSPRVSSDPRADLDSAVLLTR SLLADTRQLAAQMRDKFPADGDHSL DSLPTLAMSAGTLGSLQLPGVLTRL RVDLMSYLRHVQWLRRAGGPSLKTL EPELGALQARLERLLRRLQLLMSRL ALPQAAPDQPVIPLGPPASAWGSIR AAHAILGGLHLTLDWAVRGLLLLKT RL |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Murine Interleukin-11 (IL-11) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Murine Interleukin-11 (IL-11) should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
