Recombinant Human IL11 293 Cell Lysate

• Cat.No.: IL11-5249HCL

Specification

• Description: Antigen standard for interleukin 11 (IL11) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: IL11 interleukin 11 [ Homo sapiens ]
• Official Symbol: IL11
• Synonyms: IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809
• Chromosome Location: 19q13.3-q13.4
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Rheumatoid arthritis, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-11 receptor binding

Reviews

10/28/2019

Potent in bone regeneration models.

11/26/2017

Suitable for cell culture.

01/10/2017

High yield in expression.

Q&As

How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis?

Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.

Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA?

Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.

What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms?

Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.

Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways?

Proteomics identifies IL11-interacting proteins and potential cross-talk partners.

How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation?

Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.

What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types?

RNA sequencing uncovers IL11-induced gene expression changes across different cell types.

What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex?

X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.

How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues?

Confocal and super-resolution microscopy visualize IL11 localization in tissues.

How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA?

Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.

What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling?

CRISPR screening unveils genes and pathways influenced by IL11 signaling.