Active Recombinant Mouse Il13ra2 protein(Met1-Lys334), His&hFc-tagged

• Cat.No.: Il13ra2-764M
• Product Overview: Recombinant Mouse IL13Rα2 (NP_032382.1)(Met 1-Lys 334) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: Met1-Lys334
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized human IL13 at 20 μg/ml (100 μl/well) can bind mouse IL13RA2 with a linear range of 0.5-2 μg/ml. 2. Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/ml.
• Molecular Mass: The recombinant mouse IL13Rα2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 561 amino acids and has a predicted molecular mass of 64.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIL13Rα2/Fc monomer is approximately 75-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il13ra2 interleukin 13 receptor, alpha 2 [ Mus musculus ]
• Official Symbol: Il13ra2
• Synonyms: IL13RA2; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; IL-13RA2; IL-13R-alpha-2; IL-13R subunit alpha-2; IL-13 receptor subunit alpha-2; CD213a2
• Gene ID: 16165
• mRNA Refseq: NM_008356
• Protein Refseq: NP_032382