Recombinant Human IL13RA2 cell lysate

• Cat.No.: IL13RA2-2921HCL
• Product Overview: Human IL13RA2 / IL13R derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL13RA2 interleukin 13 receptor, alpha 2 [ Homo sapiens ]
• Official Symbol: IL13RA2
• Synonyms: IL13RA2; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; cancer/testis antigen 19; CD213a2; CT19; IL 13R; IL13BP; IL-13RA2; IL-13R-alpha-2; IL-13R subunit alpha-2; IL-13 receptor subunit alpha-2; interleukin 13 binding protein; interleukin-13-binding protein; interleukin 13 receptor alpha 2 chain; IL-13R; CD213A2
• Gene ID: 3598
• mRNA Refseq: NM_000640
• Protein Refseq: NP_000631
• MIM: 300130
• UniProt ID: Q14627
• Chromosome Location: Xq13.1-q28
• Pathway: IL4-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine receptor activity; receptor activity; signal transducer activity

Reviews

07/14/2019

Reliable in co-immunoprecipitation.

09/24/2018

High specificity.

01/05/2017

Effective in asthma models.

Q&As

What experimental methods determine IL13RA2's role in cancer metastasis?

Methods unveil IL13RA2's role in cancer metastasis.

How does proximity ligation assay reveal IL13RA2 interactions with other proteins?

Proximity ligation assay exposes IL13RA2 interactions with proteins.

Can you elaborate on the use of immunohistochemistry in studying IL13RA2 expression in tissues?

Immunohistochemistry maps IL13RA2 expression in tissues.

How do structural studies such as X-ray crystallography inform IL13RA2's ligand binding mechanism?

Structural studies like X-ray crystallography illuminate IL13RA2's ligand binding mechanism.

What are the advantages of using siRNA libraries to explore IL13RA2 functions?

siRNA libraries probe diverse IL13RA2 functions advantageously.

Could you describe how CRISPR screening identifies regulators of IL13RA2 expression?

CRISPR screening identifies regulators of IL13RA2 expression.

What techniques investigate IL13RA2 dimerization in cellular contexts?

Techniques reveal IL13RA2 dimerization in cellular settings.

Could you explain how mass spectrometry identifies IL13RA2 post-translational modifications?

Mass spectrometry detects IL13RA2 post-translational modifications.

How does surface plasmon resonance (SPR) analyze IL13RA2 binding affinities?

SPR quantifies IL13RA2 binding affinities with precision.

How do reporter gene assays assess IL13RA2-mediated signaling pathways?

Reporter gene assays illuminate IL13RA2-mediated signaling.