Active Recombinant Mouse Il2 Protein

• Cat.No.: Il2-106M
• Product Overview: Recombinant mouse IL2 (21-169aa) protein without tag was expressed in E.coli.

Specification

• Description: IL2(Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.
• Source: E. coli
• Species: Mouse
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using CTLL-2 mouse T lymphocyte. The ED50 for this effect is < 0.9 ng/mL.
• Molecular Mass: 17.3kDa
• AA Sequence: MAPTSSSTSSSTAEAQQQQQQQQQQ QQHLEQLLMDLQELLSRMENYRNLK LPRMLTFKFYLPKQATELKDLQCLE DELGPLRHVLDLTQSKSFQLEDAEN FISNIRVTVVKLKGSDNTFECQFDD ESATVVDFLRRWIAFSQSIISTSPQ
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by BCA assay)
• Storage Buffer: In Phosphate Buffered Saline (pH7.4), 10% glycerol

Gene Information

• Gene Name: Il2
• Official Symbol: Il2 interleukin 2 [ Mus musculus (house mouse) ]
• Synonyms: Il2; interleukin 2; Il-2; interleukin-2; T-cell growth factor; TCGF
• Gene ID: 16183
• mRNA Refseq: NM_008366
• Protein Refseq: NP_032392
• UniProt ID: P04351

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.