Recombinant Mouse Interferon Beta 1, Fibroblast, Fc-tagged

• Cat.No.: Ifnb1-177M
• Product Overview: A DNA sequence encoding the extracellular domain of mouse Ifnb1 was fused with the Fc region of human IgG1 at the C-terminus. The secreted recombinant mouse Ifnb1 is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a calculated molecular mass of 46.7 kDa.

Specification

• Description: Ifnb1 is one type of IFN beta proteins that is a member of the Interferon type I family. Ifnb1 is produced in large quantities by fibroblasts and binds to a specific cell surface receptor complex known as the IFN alpha receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains as other members of IFN1 famlily do. The induction of interferon beta (Ifnb1) is a key event in the antiviral immune response. It has shown that treatment with IFN beta affects NK cell cycle without altering NK cell apoptosis in MS patients. Treated with IFN beta alone or in combination with low-dose prednisolone displayed increased proportion of all NK cell subsets in the active phase of the cell cycle. There was no difference in NK cell apoptosis markers. Synthetic interferons (IFNs) are used in the treatment of several types of cancer. It has also been proved that apoptosis was decreased by IFN treatment, whereas inhibition of proliferation is caused by cell cycle arrest in late S-phase.
• Source: Human Cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Ile 22
• Form: Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Molecular Mass: The secreted recombinant mouse Ifnb1 is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a calculated molecular mass of 46.7 kDa. As a result of glycosylation, the apparent molecular mass of rmIfnb1 monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.
• Protein length: 402 amino acids
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >92 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Ifnb1 interferon beta 1, fibroblast [ Mus musculus ]
• Official Symbol: Ifnb1
• Synonyms: IFNB1; interferon beta 1, fibroblast; interferon beta; Ifb; IFNB; IFN-beta
• Gene ID: 15977
• mRNA Refseq: NM_010510
• Protein Refseq: NP_034640
• Pathway: Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (Bi°Carta), organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem
• Function: cytokine activity; cytokine receptor binding; transcription corepressor activity