| Source : |
HEK293 |
| Species : |
Rat |
| Tag : |
His |
| Form : |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : |
1. Immobilized rat IL21R-His at 10 μg/ml (100 μl/well) can bind biotinylated rat IL21-His, The EC50 of biotinylated rat IL21-His is 4.6-10.9 ng/ml.2. Immobilized Recombinant Rat IL-21 Protein at 2μg/mL (100μL/well) can bind Recombinant Rat IL-21R / Interleukin-21 Receptor Protein (His Tag), the EC50 is 80-320 ng/mL. |
| Molecular Mass : |
The recombinant rat IL21R comprises 228 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Protein Length : |
Met1-Pro236 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
