| Cat.No. : |
WNT8A.L-1597X |
| Product Overview : |
Recombinant Xenopus laevis (African clawed frog) WNT8A.L Protein (23-358 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein. |
| Description : |
Ligand for mbers of the frizzled family of seven transmbrane receptors. Plays a role in ventral mesodermal patterning during bryogenesis. Mimics Nieuwkoop center activity. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with noggin and nuclear, sequence-specific DNA-binding protein xBra. None of these molecules causes dorsal mesoderm formation when expressed alone. |
| Source : |
Yeast |
| Species : |
Xenopus laevis |
| Tag : |
His |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
39.7 kDa |
| Protein length : |
23-358 aa |
| AA Sequence : |
AWSVNNFLMT GPKAYLTYSA SVAVGAQNGI EECKYQFAWE RWNCPESTLQ LATHNGLRSA TRETSFVHAI SSAGVMYTLT RNCSMGDFDN CGCDDSRNGR IGGRGWVWGG CSDNAEFGER ISKLFVDGLE TGQDARALMN LHNNEAGRLA VKETMKRTCK CHGISGSCSI QTCWLQLAEF RDIGNHLKIK HDQALKLEMD KRKMRSGNSA DNRGAIADAF SSVAGSELIF LEDSPDYCLK NISLGLQGTE GRECLQSGKN LSQWERRSCK RLCTDCGLRV EEKKTEIISS CNCKFHWCCT VKCEQCKQVV IKHFCARRER DSNMLNTKRK NRGHRR |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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