What is CLCF1 Protein

      CLCF1, or Cytokine-Like Factor 1, is a protein encoded by the CLCF1 gene. Also known by the aliases BSF3, Novel neurotrophin-1/B cell-stimulating factor-3, and NNT-1/BSF-3, CLCF1 is a member of the interleukin-6 (IL-6) family of cytokines. This family includes prominent signaling molecules involved in regulating immune responses, inflammation, and hematopoiesis.

      CLCF1 Protein Structural Characteristics and Classification

      Structurally, CLCF1 exhibits the typical characteristics of the IL-6 cytokine family, featuring a four-helix bundle structure. Recent advances in research have shed light on the classification of CLCF1 as a neurotrophic factor, highlighting its role in the development and maintenance of the nervous system. The identification of its distinct structural attributes has facilitated a deeper understanding of its functional implications.

      CLCF1 Biological Functions and Molecular Mechanisms

      CLCF1 plays a pivotal role in various biological processes, orchestrating cellular responses crucial for homeostasis. At its core, CLCF1 operates as a neurotrophic factor, promoting the survival and differentiation of neuronal cells. Its influence extends beyond the nervous system, contributing to the regulation of immune responses and hematopoiesis.

      Molecularly, CLCF1 exerts its effects by binding to the ciliary neurotrophic factor receptor (CNTFR), which forms a complex with the leukemia inhibitory factor receptor (LIFR) and glycoprotein 130 (gp130). This intricate interplay activates downstream signaling cascades, including the JAK/STAT pathway, ultimately modulating gene expression and cellular responses.

      Figure 1. Diagram of the role and mechanism of CLCF1 in promoting the whitening of brown adipose tissue. (Ding M, et al., 2023)

      Figure 1. Diagram of the role and mechanism of CLCF1 in promoting the whitening of brown adipose tissue. (Ding M, et al., 2023)

      CLCF1 Related Signaling Pathway

      The signal pathway associated with CLCF1 is intricately connected to its receptor interactions. Upon binding to the CNTFR/LIFR/gp130 complex, CLCF1 activates the JAK/STAT pathway, initiating a series of phosphorylation events that culminate in the transcriptional regulation of target genes. This pathway serves as a molecular bridge between extracellular signals and intracellular responses, dictating cellular fate and function.

      CLCF1 Related Diseases

      While CLCF1's role in normal physiological processes is well-established, dysregulation of its expression or signaling pathways can contribute to the development of various diseases. Notably, CLCF1 has been implicated in neurodegenerative disorders, where aberrant signaling may lead to neuronal dysfunction and degeneration. Additionally, disruptions in the immune and hematopoietic systems have been linked to CLCF1-related diseases, highlighting its diverse impact on health.

      CLCF1's Applications in Biomedicine

      Beyond its role in understanding physiological and pathological processes, CLCF1 holds significant promise in various biomedical applications. Its unique properties make it an attractive candidate for diagnostic development, where its detection could serve as a biomarker for certain diseases. Moreover, the neurotrophic and immunomodulatory functions of CLCF1 position it as a potential player in vaccine development, harnessing its capabilities to enhance immune responses.

      Therapeutically, CLCF1 opens avenues for novel treatment strategies. Manipulating its signaling pathways may offer targeted interventions in neurodegenerative disorders, immune-related diseases, and hematopoietic disorders. As research advances, the translation of CLCF1-based therapies from bench to bedside becomes an exciting prospect in the realm of precision medicine.

      Recommended Products

      Cat.# Product name Species Source (Host) Tag
      CLCF1-30449TH Recombinant Human CLCF1 protein Human E.coli N/A
      CLCF1-953H Recombinant Human Cardiotrophin-like Cytokine Factor 1 Human E.coli N/A
      CLCF1-11277H Recombinant Human CLCF1, His-tagged Human E.coli His
      CLCF1-29299TH Recombinant Human CLCF1, His-tagged Human E.coli His
      CRLF1&CLCF1-1036H Active Recombinant Human CRLF1&CLCF1 Human Mammalian cells N/A
      CLCF1-3242H Recombinant Human CLCF1 Protein, MYC/DDK-tagged Human HEK293 Myc/DDK
      CLCF1-5167H Recombinant Human CLCF1 Protein, Myc/DDK-tagged, C13 and N15-labeled Human HEK293T Myc/DDK
      CLCF1-351H Recombinant Human CLCF1, Fc-tagged Human Human Cell Fc
      CLCF1-618H Recombinant Human CLCF1 Protein, His-tagged Human E.coli His
      CLCF1-2065H Recombinant Human CLCF1 Protein (Leu28-Phe225), N-His tagged Human E.coli N-His

      Reference

      • Ding M, et al. CLCF1 signaling restrains thermogenesis and disrupts metabolic homeostasis by inhibiting mitochondrial biogenesis in brown adipocytes. Proceedings of the National Academy of Sciences. 2023, 120(33): e2305717120.