ARF4
-
Official Full Name
ADP-ribosylation factor 4
-
Overview
This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. -
Synonyms
ARF4; ADP-ribosylation factor 4; ADP ribosylation factor 2; ADP ribosylation factor 4; ARF2; ADP-ribosylation factor 2;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Human
- Mouse
- Rat
- Rhesus Macaque
- E.coli
- E.Coli or Yeast
- HEK293
- HEK293T
- In Vitro Cell Free System
- Mammalian Cell
- Wheat Germ
- GST
- His
- His (Fc)
- Avi
- Myc
- DDK
- MYC
- N/A
- Involved Pathway
- Protein Function
- Interacting Protein
- ARF4 Related Signal Pathway
ARF4 involved in several pathways and played different roles in them. We selected most pathways ARF4 participated on our site, such as Assembly of the primary cilium, Cargo trafficking to the periciliary membrane, EGFR1 Signaling Pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with ARF4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
---|---|
Assembly of the primary cilium | CEP72;CEP135;C11orf60;DYNLL2B;BBS12;TCTEX1D1;FBF1;DCTN1A;ODF2 |
Cargo trafficking to the periciliary membrane | BBS12;PKD1;LZTFL1;EXOC1;NPHP3;ARF4A;EXOC6;MKKS;EXOC3 |
EGFR1 Signaling Pathway | EEF1A1;GRB7;KLF11;JUND;RBBP7;USP6NL;GRB10;PLSCR1;TNK2 |
ErbB1 downstream signaling | MAP3K2;BAIAP2;EGR1;ACTR3;CHN2;ABI1;EPS8;WASF2;ARF4 |
Organelle biogenesis and maintenance | GABPA;MRPL42;IFT20;IFT-20;GABPB1;UNC119.1;MRPL46;PPRC1;TFB2M |
VxPx cargo-targeting to cilium | CNGA5;PKD1;ARF4;CNGA2;PKD2;ARF4A;RAB3IP |
ARF4 has several biochemical functions, for example, GTP binding, GTPase activity, epidermal growth factor receptor binding. Some of the functions are cooperated with other proteins, some of the functions could acted by ARF4 itself. We selected most functions ARF4 had, and list some proteins which have the same functions with ARF4. You can find most of the proteins on our site.
Function | Related Protein |
---|---|
GTP binding | GBP2;DIRAS1;ARL1;RAB11A;RHOGB;GEM;MX2;RAB3AA;ARL4CA |
GTPase activity | GTPBP1;TUBB2B;GBP2;GIMAP7;GNA13;EEF2A.1;RAN;TUBB2A;GSPT1L |
epidermal growth factor receptor binding | LINGO1B;MS4A1;SNX1;EPGN;RNF126;YES1;TNK2;EREG;ERBB4 |
protein binding | FAM161A;CDH15;ZW10;IL15RA;ULK1;CAMLG;DCC;Cd24a;PSMC5 |
ARF4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ARF4 here. Most of them are supplied by our site. Hope this information will be useful for your research of ARF4.
SDCBP2; RCHY1; Cdk1; BABAM1; pi3p; 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate; MYC; ATF2; STK4; SNW1; AGO4
- Q&As
- Reviews
Q&As (14)
Ask a questionARF4 plays a vital role in membrane remodeling events by recruiting various coat proteins, such as COPI and clathrin, to the appropriate membrane domains. This recruitment facilitates membrane curvature and vesicle formation, allowing for the remodeling and trafficking of intracellular membranes.
Currently, there are no specific diseases or disorders directly linked to ARF4 mutations or dysregulation in humans. However, studies have shown that ARF4 dysregulation can contribute to the pathogenesis of certain diseases, such as neurodegenerative disorders like Alzheimer's disease and Huntington's disease, where aberrant vesicle trafficking and protein misfolding are involved.
Current research on ARF4 is focused on understanding its precise molecular mechanisms and its roles in various cellular processes. This includes investigating its interactions with binding partners, its involvement in vesicle trafficking, protein sorting, and membrane dynamics, as well as its contributions to disease pathogenesis, especially in cancer and neurodegenerative disorders.
To my knowledge, there are no specific small molecule inhibitors or activators of ARF4 currently available. However, there have been efforts to develop compounds that modulate ARF family proteins in general for therapeutic purposes. These compounds often target small GTPases in general or specific regulators of ARF proteins rather than directly acting on ARF4 itself.
Yes, ARF4 has been implicated in maintaining cellular homeostasis and responding to stress conditions. It participates in the regulation of organelle integrity and protein sorting during cellular stress, ensuring proper cellular function and adaptation to environmental challenges.
ARF4 has been implicated in various aspects of cancer biology. It plays a role in tumor cell migration and invasion by regulating the trafficking of proteins involved in cell adhesion and cytoskeletal rearrangement. ARF4 also regulates the secretion of growth factors and cytokines, which can promote tumor growth and angiogenesis. Additionally, studies have shown that ARF4 can modulate tumor cell sensitivity to chemotherapy agents, suggesting its involvement in drug resistance mechanisms.
Yes, ARF4 can interact with several binding partners, including various coat proteins like AP-1, AP-3, and AP-4, which are involved in vesicle formation and cargo sorting. It can also interact with proteins involved in membrane fusion and tethering, such as SNARE proteins and tethering factors like golgins.
Yes, ARF4 has been implicated in cell migration and adhesion processes. It is involved in the regulation of membrane protrusions at the leading edge of migrating cells, facilitating cell movement. ARF4 also regulates the trafficking and recycling of adhesion molecules, affecting cell adhesion dynamics.
ARF4 plays a crucial role in protein sorting at the Golgi by recruiting coat protein complexes and regulating the formation of transport vesicles. It helps in the packaging of cargo proteins into specific vesicles for transport to their target compartments within the cell.
Yes, studies in animal models have shown that ARF4 is essential for embryonic development. Complete loss of ARF4 in mice is embryonically lethal, indicating its critical role in fundamental cellular processes required for proper development.
Currently, there is limited information on genetic variants or mutations specifically in the ARF4 gene associated with human diseases. However, dysregulation of ARF4 or its related proteins has been linked to disorders such as cancer, neurodegenerative diseases, and various developmental abnormalities.
Targeting ARF4 or its associated proteins could have potential therapeutic implications. Modulating ARF4 activity or its specific downstream effectors may help regulate cellular processes affected in various diseases, including cancer, neurodegenerative disorders, and certain genetic diseases associated with vesicle trafficking defects. However, further research is needed to fully understand ARF4's potential as a therapeutic target.
Although ARF4's primary role is in vesicular trafficking, it can indirectly influence signaling pathways by regulating the transport and localization of signaling molecules and receptors within the cell. For example, ARF4 has been shown to play a role in the recycling of certain receptors, thus impacting their signaling activity.
Yes, ARF4 can interact with numerous proteins and regulatory factors to carry out its cellular functions. These include various coat protein complexes (e.g., COPI, clathrin), GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs), and effector proteins involved in vesicular trafficking and membrane dynamics.
Customer Reviews (8)
Write a reviewWhether it be troubleshooting issues, offering experimental guidance, or addressing any concerns, the manufacturer's technical support ensures that researchers have a reliable and knowledgeable resource to rely on.
From recombinant protein production to modification or labeling services, the company is committed to assisting researchers in obtaining the Arg2 protein in the format and specifications required for their particular studies.
the manufacturer understands the importance of customization and can offer tailored solutions to meet specific research needs.
The manufacturer of the ARF4 protein plays a vital role in supporting my trials.
The company provides a dedicated team of experts who are readily available to assist researchers throughout their experimental journey.
the ARF4 protein not only fulfills my experimental requirements but is also backed by excellent technical support, which guarantees a smooth and successful research experience.
Whether I am investigating enzymatic activity, studying protein-protein interactions, or testing its role in signaling pathways, the ARF4 protein consistently performs exceptionally well.
By using the ARF4 protein in my experiments, I am confident in obtaining accurate and dependable data.
Ask a Question for All ARF4 Products
Required fields are marked with *
My Review for All ARF4 Products
Required fields are marked with *