SPARCL1
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Official Full Name
SPARC-like 1 (hevin)
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Synonyms
SPARCL1; SPARC-like 1 (hevin); SPARC like 1 (mast9, hevin); SPARC-like protein 1; MAST9; Ecm2; RAGS1 antigen; Extracellular matrix protein 2; Hevin; High endothelial venule protein; MAST 9; Matrix glycoprotein Sc1; PIG33; proliferation inducing protein 33; SC1; SPARC like 1; SPRL1_HUMAN; SPARC-like 1 (mast9, hevin); proliferation-inducing protein 33;
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What is SPARCL1 protein?
SPARCL1 gene is a protein coding gene which situated on the long arm of chromosome 4 at locus 4q.22 that spans 57.7 Kb gDNA. SPARCL1 (Secreted Protein, Acidic and Rich in Cysteineslike 1), also known as hevin, SC1 or MAST9 is encoded by the SPARCL1 gene. It is a member of the SPARC family of extracellular glycoproteins that contained 664 amino acids (aa). Its molecular mass is approximately 75 kDa but migrates at ~130 kDa, which has been explained either by disulfidelinked homodimerization or by glycosylation and high acidity. The subcellular locations for SPARCL1 is usually secreted, or in extracellular space and extracellular matrix. SPARCL1 is an antiadhesive protein that is highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. Human SPARCL1 contains a 16 aa signal sequence and a 648 aa mature region with four domains: a 416 aa Nterminal acidic region, a 23 aa follistatinlike domain, a 55 aa kazallike segment and a 48 aa EFhand/calciumbinding domain. Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72% and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatinlike, kazallike and calciumbinding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC.
What is the function of SPARCL1 protein?
SPARCL1 inhibits adhesion and spreading on a variety of substrate. It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. SPARCL1 specifically promotes the formation and maintenance of thalamocortical excitatory synapses, by linking presynaptic neurexin-1α and postsynaptic neuroligin-1B. Importantly, SPARCL1 has been implicated in resilience to stress, showing an antidepressant-like effect in a model of social defeat. Also, SPARCL1 is one of the most downregulated transcripts in the frontopolar cortex of depressed/suicide victims. In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation. SPARCL1 binds collagen in mice, deletion of SPARCL1 causes dermal collagen fibrils that are smaller in diameter and deficient in decorin.
SPARCL1 Related Diseases
Neurodegenerative Disorders. Downregulated SPARCL1 has been found in the cerebrospinal fluid (CSF) of individuals with Parkinson´s and Alzheimer´s diseases.
Emotional Control Disorder. SPARCL1 has been implicated in resilience to stress, also, SPARCL1 is one of the most downregulated transcripts in the frontopolar cortex of depressed/suicide victims. Tumors. SPARCL1 is a matricellular protein with anti-adhesive, anti-proliferative and anti-tumorigenic functions and is frequently downregulated in tumors such as colorectal carcinoma or non-small cell lung cancer.
Fig1. SPARCL1 expression in BC tissues and matched normal adjacent breast tissues (Wilcoxon matched-pairs signed-rank test).
Biomedical Application of SPARCL1 Protein
SPARCL1 has also been looked at as a potential biomarker of neurodegenerative disorders and has been found in the cerebrospinal fluid (CSF) of individuals with Parkinson´s and Alzheimer´s diseases. Also, SPARCL1 is a novel prognostic biomarker and correlates with tumor microenvironment in colorectal cancer or prostate cancer.One research reavealed SPARCL1 could act as a biomarker of maladaptive right ventricular remodelling in pulmonary hypertension.
High Purity
Fig1. SDS-PAGE (SPARCL1-6625H) (PROTOCOL for western blot)
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Fig2. SDS-PAGE (SPARCL1-2466H)
Case Study 1: Amaia Nuñez-delMoral, 2021
Fig1. Protein dependent-curve of hevin/SPARCL1 immunoreactivity in prefrontal cortex total homogenate pool (5-60 μg) with goat anti-human hevin antibody (and the corresponding quantification of ~130 kDa and ~100 kDa hevin bands).
Case Study 2: Anika Klingler, 2020
Fig2. Western blot analysis of Murine Sparcl1 protein expression in different murine organs of Sparcl1 wild-type (Sc1 +/+, n = 3) and knockout (Sc1 -/-, n = 3) mice.
SPARCL1 involved in several pathways and played different roles in them. We selected most pathways SPARCL1 participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with SPARCL1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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SPARCL1 has several biochemical functions, for example, calcium ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SPARCL1 itself. We selected most functions SPARCL1 had, and list some proteins which have the same functions with SPARCL1. You can find most of the proteins on our site.
Function | Related Protein |
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calcium ion binding | PKD1L2;CDH1;DSG1;MMP1;PLA2G4B;EML1;MGP;SPNA2;CDH2 |
SPARCL1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SPARCL1 here. Most of them are supplied by our site. Hope this information will be useful for your research of SPARCL1.
TNF; CBFB; PDGFRL
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Customer Reviews (3)
Write a reviewThe bands of proteins in Western Blotting experiments were very clear.
Researchers can rely on the SPARCL1 protein to deliver precise results, enhancing the credibility and efficiency of their experiments.
The SPARCL1 protein is highly recommended for its exceptional performance in ELISA assays.
Q&As (5)
Ask a questionSPARCL1 interacts with cell adhesion molecules and the extracellular matrix, influencing cell adhesion and migration in various physiological processes.
SPARCL1 is involved in tissue repair and wound healing processes, influencing cell migration, adhesion, and extracellular matrix remodeling.
Studies suggest that low SPARCL1 expression is associated with poor prognosis in certain cancers, indicating its potential as a predictive marker.
Modulating SPARCL1 expression could be a potential therapeutic strategy, particularly in cancer treatment, by targeting tumor progression or metastasis.
SPARCL1 is frequently downregulated in breast, lung, and colorectal cancers, suggesting a potential role as a prognostic marker.
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