Active Native Bovine α-Thrombin

• Cat.No.: F2-274B

Specification

• Species: Bovine
• Source: Plasma
• Tag: Non
• Description: Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin. During coagulation, thrombin cleaves fibrinogen to form fibrin, leading to the ultimate step in coagulation, the formation of a fibrin clot. Thrombin is also responsible for feedback activation of the procofactors factor V and factor VIII. Thrombin has also been reported to activate factor XIII and platelets, and also functions as a vasoconstrictor protein. The procoagulant activity of thrombin is arrested in two ways: 1) inhibition by either heparin cofactor II or the antithrombin III/heparin complex; or 2) complex formation with thrombomodulin. Formation of the thrombin/thrombomodulin complex results in the inability of thrombin to cleave fibrinogen and activate factors V and VIII, but increases the efficiency of thrombin for activation of the anticoagulant, protein C.
• Form: 50% glycerol/water (v/v)
• Bio-activity: Fibrinogen clotting or chromogenic assay
• Molecular Mass: 36700
• Purity: >95% by SDS-PAGE. NOT tissue/cell culture grade. Not tested for endotoxin.
• Characteristic: Extinction coefficient:19.5, Percent carbohydrate: approximately 5%, Structure: two subunits, approximately Mr=6,000 and 31,000
• Storage: -20°C

Gene Information

• Gene Name: F2?coagulation factor II (thrombin) [?Bos taurus?(cattle) ]
• Official Symbol: F2
• Synonyms: prothrombin; thrombin
• Gene ID: 280685
• mRNA Refseq: NM_173877
• Protein Refseq: NP_776302
• UniProt ID: P00735
• Chromosome Location: chromosome: 15
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem
• Function: calcium ion binding; fibrinogen binding