| Species : |
Clostridium botulinum |
| Source : |
E.coli |
| Protein Length : |
1-425 a.a. |
| Description : |
Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A2 which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. |
| Bio-activity : |
Measured by its ability to cleave the fluorogenic peptide substrate, SNAPtide. The specific activity is >1 pmol/min/μg, as measured under the described conditions. |
| Molecular Mass : |
50 kDa |
| Product-Related Proteins : |
1-425 |
| Endotoxin : |
< 0.1 EU/μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
At -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : |
PBS |
