Active Recombinant Cynomolgus FCGRT/B2M protein, His/Avi-tagged, Biotinylated
Cat.No. : | FCGRT&B2M-1536C |
Product Overview : | Recombinant Cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; Recombinant cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
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Source : | HEK293 |
Species : | Cynomolgus |
Tag : | His/Avi |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized rhFc(CHO) at 10 μg/mL (100 μL/well) can bind cynoFCGRT-Avih, the EC50 of cynoFCGRT-Avih is 400-900 ng/mL. |
Molecular Mass : | The recombinant cynomolgus FCGRT&B2M consists of 399 (300+99) amino acids and predicts a molecular mass of 45.3 (33.7+ 11.6) KDa. |
Protein length : | Met 1-Ser 297 & Met 1-Met 119 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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