Acitve Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-1373H
• Product Overview: Recombinant Human FCGRT (P55899)(Met1-Ser297) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified and expressed in HEK293. The purified protein was biotinylated in vitro.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser297 & Met1-Met119
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 4.6 nM.
• Molecular Mass: The recombinant heterodimer of Human FCGRT/B2M consists of 385 (285 + 100) amino acids and predicts a molecular mass of 43.5 (31.8 + 11.7) kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
• Official Symbol: FCGRT; B2M
• Synonyms: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain
• Gene ID: 2217
• mRNA Refseq: NM_001136019
• Protein Refseq: NP_001129491
• MIM: 601437
• UniProt ID: P55899