Acitve Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged, Biotinylated
Cat.No. : | FCGRT & B2M-1373H |
Product Overview : | Recombinant Human FCGRT (P55899)(Met1-Ser297) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified and expressed in HEK293. The purified protein was biotinylated in vitro. |
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Source : | HEK293 |
Species : | Human |
Tag : | C-His |
Protein length : | Met1-Ser297 & Met1-Met119 |
Conjugation/Label : | Biotin |
Form : | Lyophilized from sterile PBS. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 4.6 nM. |
Molecular Mass : | The recombinant heterodimer of Human FCGRT/B2M consists of 385 (285 + 100) amino acids and predicts a molecular mass of 43.5 (31.8 + 11.7) kDa. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ] |
Official Symbol : | FCGRT; B2M |
Synonyms : | FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain; |
Gene ID : | 2217 |
mRNA Refseq : | NM_001136019 |
Protein Refseq : | NP_001129491 |
MIM : | 601437 |
UniProt ID : | P55899 |
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◆ Lysates | ||
FCGRT-6278HCL | Recombinant Human FCGRT 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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