Active Recombinant Full Length Human FGR Protein, C-Flag-tagged

• Cat.No.: FGR-898HFL
• Product Overview: Recombinant Full Length Human FGR Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: FGR activity verified in a biochemical assay: FGR (Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FGR is a tyrosine kinase that is a member of the Src family of protein tyrosine kinases. Varying concentrations of FGR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 59.3 kDa
• AA Sequence: MGCVFCKKLEPVATAKEDAGLEGDF RSYGAADHYGPDPTKARPASSFAHI PNYSNFSSQAINPGFLDSGT IRGVSGIGVTLFIALYDYEARTEDD LTFTKGEKFHILNNTEGDWWEARSL SSGKTGCIPSNYVAPVDSIQ AEEWYFGKIGRKDAERQLLSPGNPQ GAFLIRESETTKGAYSLSIRDWDQT RGDHVKHYKIRKLDMGGYYI TTRVQFNSVQELVQHYMEVNDGLCN LLIAPCTIMKPQTLGLAKDAWEISR SSITLERRLGTGCFGDVWLG TWNGSTKVAVKTLKPGTMSPKAFLE EAQVMKLLRHDKLVQLYAVVSEEPI YIVTEFMCHGSLLDFLKNPE GQDLRLPQLVDMAAQVAEGMAYMER MNYIHRDLRAANILVGERLACKIAD FGLARLIKDDEYNPCQGSKF PIKWTAPEAALFGRFTIKSDVWSFG ILLTELITKGRIPYPGMNKREVLEQ VEQGYHMPCPPGCPASLYEA
  MEQTWRLDPEERPTFEYLQSFLEDY FTSAEPQYQPGDQTTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway

Gene Information

• Gene Name: FGR FGR proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: FGR
• Synonyms: SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• Gene ID: 2268
• mRNA Refseq: NM_005248.3
• Protein Refseq: NP_005239.1
• MIM: 164940
• UniProt ID: P09769