Active Recombinant Full Length Human POT1 Protein, C-Flag-tagged

• Cat.No.: POT1-141HFL
• Product Overview: Recombinant Full Length Human POT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
  Binding assay (competitor)
  Pull-down assay
• Molecular Mass: 71.3 kDa
• AA Sequence: MSLVPATNYIYTPLNQLKGGTIVNV YGVVKFFKPPYLSKGTDYCSVVTIV DQTNVKLTCLLFSGNYEALP IIYKNGDIVRFHRLKIQVYKKETQG ITSSGFASLTFEGTLGAPIIPRTSS KYFNFTTEDHKMVEALRVWA STHMSPSWTLLKLCDVQPMQYFDLT CQLLGKAEVDGASFLLKVWDGTRTP FPSWRVLIQDLVLEGDLSHI HRLQNLTIDILVYDNHVHVARSLKV GSFLRIYSLHTKLQSMNSENQTMLS LEFHLHGGTSYGRGIRVLPE SNSDVDQLKKDLESANLTANQHSDV ICQSEPDDSFPSSGSVSLYEVERCQ QLSATILTDHQYLERTPLCA ILKQKAPQQYRIRAKLRSYKPRRLF QSVKLHCPKCHLLQEVPHEGDLDII FQDGATKTPVVKLQNTSLYD SKIWTTKNQKGRKVAVHFVKNNGIL PLSNECLLLIEGGTLSEICKLSNKF NSVIPVRSGHEDLELLDLSA PFLIQGTIHHYGCKQCSSLRSIQNL NSLVDKTSWIPSSVAEALGIVPLQY VFVMTFTLDDGTGVLEAYLM DSDKFFQIPASEVLMDDDLQKSVDM IMDMFCPPGIKIDAYPWLECFIKSY NVTNGTDNQICYQIFDTTVA
  EDVITRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: POT1 protection of telomeres 1 [ Homo sapiens (human) ]
• Official Symbol: POT1
• Synonyms: GLM9; CMM10; HPOT1
• Gene ID: 25913
• mRNA Refseq: NM_015450.3
• Protein Refseq: NP_056265.2
• MIM: 606478
• UniProt ID: Q9NUX5