Active Recombinant Human ANGPT1 Protein, His-tagged, Biotinylated
Cat.No. : | ANGPT1-157H |
Product Overview : | Recombinant Human ANGPT1(Ser20-Phe498) fused with His tag at C-terminal was expressed in NS0, Biotinylated. |
- Specification
- Gene Information
- Related Products
Description : | Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It contains a N-terminal coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region. Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into disulfide-linked homotrimers, tetramers, and pentamers. Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation. Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels. In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell. In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum. In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin. Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2. These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis. |
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ser20 |
Form : | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein. |
Bio-activity : | Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 μg/mL of a cross-linking an |
Molecular Mass : | 56 kDa (unlabeled) |
Protein length : | Ser20-Phe498 |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >90%, by SDS-PAGE with silver staining |
Notes : | Structure / Form: Oligomer |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile |
Reconstitution : | Reconstitute at 10 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name : | ANGPT1 angiopoietin 1 [ Homo sapiens ] |
Official Symbol : | ANGPT1 |
Synonyms : | ANGPT1; angiopoietin 1; angiopoietin-1; Ang1; KIAA0003; ANG-1; AGP1; AGPT; ANG1; |
Gene ID : | 284 |
mRNA Refseq : | NM_001146 |
Protein Refseq : | NP_001137 |
MIM : | 601667 |
UniProt ID : | Q15389 |
Products Types
◆ Recombinant Protein | ||
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ANGPT1-152R | Recombinant Rhesus Macaque ANGPT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ANGPT1-1065H | Recombinant Human ANGPT1 protein(Met1-Leu261 and Arg277-Phe498), hFc-tagged | +Inquiry |
ANGPT1-1066C | Recombinant Cynomolgus ANGPT1 Protein (Arg277-Phe498), HlgG1 Fc-tagged | +Inquiry |
ANGPT1-2466H | Recombinant Human ANGPT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
ANGPT1-8863HCL | Recombinant Human ANGPT1 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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