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Active Recombinant Human BMP2 Protein

Cat.No. : BMP2-437B
Product Overview : Recombinant Human BMP2 Protein without tag was expressed in E. coli.
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Description : Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.
Source : E. coli
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Assay #1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, The ED50 for this effect is typically 0.07-0.2 μg/mL.
Assay #2: Measured by its ability to induce alkaline phosphatase production by C2C12 cells, The ED50 for this effect is typically 0.2-1 μg/mL.
Molecular Mass : 26 kDa, observed by non-reducing SDS-PAGE
AA Sequence : MQAKHKQRKRLKSSCKRHPLYVDFS DVGWNDWIVAPPGYHAFYCHGECPF PLADHLNSTNHAIVQTLVNSVNSKI PKACCVPTELSAISMLYLDENEKVV LKNYQDMVVEGCGCR
Endotoxin : < 1 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by non-reducing SDS-PAGE.
Storage : Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against 50 mM acetic acid.
Reconstitution : Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/mL.
Gene Name : BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
Official Symbol : BMP2
Synonyms : BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2;
Gene ID : 650
mRNA Refseq : NM_001200
Protein Refseq : NP_001191
MIM : 112261
UniProt ID : P12643

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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05/06/2023

    We have tested the recombinant protein in our ELISAs and it performs well.

    02/01/2023

      I'm pleased with your recombinant products. It is done well in our experiment.

      01/29/2023

        Very nice puchase experience with all of you.

        08/06/2022

          I’ve used the products you provided, and they all work good in my study.

          04/09/2021

            Thanks so much! Your protein performed really well,

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