| Description : |
Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Assay #1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, The ED50 for this effect is typically 0.07-0.2 μg/mL.
Assay #2: Measured by its ability to induce alkaline phosphatase production by C2C12 cells, The ED50 for this effect is typically 0.2-1 μg/mL. |
| Molecular Mass : |
26 kDa, observed by non-reducing SDS-PAGE |
| AA Sequence : |
MQAKHKQRKRLKSSCKRHPLYVDFS DVGWNDWIVAPPGYHAFYCHGECPF PLADHLNSTNHAIVQTLVNSVNSKI PKACCVPTELSAISMLYLDENEKVV LKNYQDMVVEGCGCR |
| Endotoxin : |
< 1 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by non-reducing SDS-PAGE. |
| Storage : |
Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against 50 mM acetic acid. |
| Reconstitution : |
Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/mL. |
