| Species : |
Human/Mouse/Rat |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
284-396 aa |
| Form : |
Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 30% ACN. Contact us for customized product form or formulation. |
| Bio-activity : |
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×10^4~4.07×10^4 units/mg. |
| Molecular Mass : |
12.78 kDa |
| Endotoxin : |
< 1 EU/μg of the protein by LAL method. |
| Purity : |
≥ 95 % as determined by SDS-PAGE. |
| Storage : |
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| Reconstitution : |
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |