Recombinant Human BMP2 protein

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Recombinant Human BMP2 protein

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Cat.No. : BMP2-1119H
Product Overview : Recombinant Human BMP2 (Ala284-Arg396) protein carried no tag and was expressed in E.coli.
Description : Human bone morphogenetic protein 2 (BMP2) is also known as human BMP-2, BMP-2, BMP2, BMP 2, h-BMP-2, rh-BMP-2, recombinant human BMP-2, recombinant BMP-2, BMP, is a member of the BMP subgroup belonging to the TGF-b superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. As implied by its name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function.
Source : E.coli
Species : Human
Predicted N Terminal : Ala284
Form : Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH2.0, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 13.0 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Ala284-Arg396
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of BMP2-1119H :

Human BMP-2, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA of BMP2-1119H :

Immobilized Human BMP-2, Tag Free at 2 μg/mL (100 μL/well) can bind Human Gremlin, Fc Tag with a linear range of 5-78 ng/mL (QC tested).

Gene Name : BMP2
Official Symbol : BMP2
Synonyms : BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
Gene ID : 650
mRNA Refseq : NM_001200
Protein Refseq : NP_001191
MIM : 112261
UniProt ID : P12643

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