Active Recombinant Human BTC Protein (80 aa)

• Cat.No.: BTC-135B
• Product Overview: Recombinant Human BTC Protein without tag was expressed in E. coli.

Specification

• Description: Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 × 10^7units/mg.
• Molecular Mass: Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.
• Protein length: 80
• AA Sequence: DGNSTRSPETNGLLCGDPEENCAAT TTQSKRKGHFSRCPKQYKHYCIKGR CRFVVAEQTPSCVCDEGYIGARCER VDLFY
• Endotoxin: Less than 1 EU/μg of rHuBetacellulin as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: BTC betacellulin [ Homo sapiens ]
• Official Symbol: BTC
• Synonyms: BTC; betacellulin; probetacellulin
• Gene ID: 685
• mRNA Refseq: NM_001729
• Protein Refseq: NP_001720
• MIM: 600345
• UniProt ID: P35070

Reviews

12/16/2022

Because of its high activity, few experiments were successful, reducing the number of our experiments.

08/08/2021

High cost performance, affordable price, good experimental effect.

01/30/2021

Excellent product, perfect for western-blot.

Q&As

Does BTC protein have potential in the treatment of metabolic diseases?

Yes, BTC protein is involved in both energy metabolism and fat metabolism, so it has some potential in the treatment of metabolic diseases.

How about BTC protein have an effect on nervous system development and function?

This protein has a role in the development and function of the nervous system to control the differentiation and growth of neurons.

What is the role of BTC protein in wound repair?

BTC protein can promote cell proliferation, pocket sac (D472250) and microvascular growth, etc., and plays an important role in wound healing and repair.

Does BTC protein have a role in PTSD treatment?

This protein plays a regulatory role in the pathogenesis and treatment mechanism of PTSD, and is one of the new targets for the treatment of Wolverine poisoning.

What is the role of BTC protein in osteoporosis?

It is involved in the occurrence and process of osteoporosis, and related research has become an important breakthrough in the treatment of osteoporosis.

What is the role of BTC protein in organ transplant rejection?

BTC is involved in the occurrence and process of organ transplant rejection and has become one of the targets for the treatment of transplant rejection.