Active Recombinant Human CA8 Protein, His-tagged
Cat.No. : | CA8-397H |
Product Overview : | Recombinant human CA8 protein fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
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Description : | CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, this protein lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). It continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). |
Source : | E.coli |
Species : | Human |
Tag : | His |
Form : | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT |
Bio-activity : | Specific activity is >450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
Molecular Mass : | 35.5 kDa (314aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher) |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ |
Purity : | >90% by SDS - PAGE |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Gene Name : | CA8 carbonic anhydrase VIII [ Homo sapiens ] |
Official Symbol : | CA8 |
Synonyms : | CA8; carbonic anhydrase VIII; CALS; carbonic anhydrase-related protein; CARP; CA-related protein; carbonate dehydratase; carbonic anhydrase-like sequence; CAMRQ3; CA-VIII; MGC99509; MGC120502; |
Gene ID : | 767 |
mRNA Refseq : | NM_004056 |
Protein Refseq : | NP_004047 |
MIM : | 114815 |
UniProt ID : | P35219 |
Chromosome Location : | 8q11-q12 |
Function : | carbonate dehydratase activity; metal ion binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
CA8-721R | Recombinant Rat CA8 Protein, His (Fc)-Avi-tagged | +Inquiry |
Car8-749M | Recombinant Mouse Car8 Protein, MYC/DDK-tagged | +Inquiry |
CA8-426R | Recombinant Rhesus Macaque CA8 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA8-2273H | Recombinant Human CA8 Protein, MYC/DDK-tagged | +Inquiry |
Car8-909M | Active Recombinant Mouse Car8 protein(Met1-Gln291), His-tagged | +Inquiry |
◆ Lysates | ||
CA8-142HCL | Recombinant Human CA8 lysate | +Inquiry |
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Not For Human Consumption!
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Q&As (7)
Ask a questionCA8 interacts with IP3Rs, affecting calcium signaling in neurons.
Unlike enzymatically active isoforms, CA8 doesn't catalyze the hydration of CO2 but instead plays roles in neuronal signaling and function.
Mutations in CA8 have been linked to cerebellar ataxia, a movement disorder.
CA8 impacts calcium signaling in neurons, affecting neural communication and potentially influencing motor control.
Specific modifications, like phosphorylation, can be explored using mass spectrometry or targeted biochemical assays.
CA8 acts as a stabilizer for inositol 1,4,5-trisphosphate receptors (IP3Rs) in neurons.
CA8 is mainly expressed in the cerebellum, particularly in Purkinje cells.
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