Species : |
Human |
Source : |
E.coli |
Protein Length : |
70 |
Description : |
MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1α chemo-attracts macrophages in order to accelerate the tissue repair process. |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
ED50 < 80 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3, corresponding to a specific activity of > 1.25 × 10^4 units/mg. |
Molecular Mass : |
7.8 kDa, observed by reducing SDS-PAGE. |
AA Sequence : |
ASLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA |
Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
Storage : |
Lyophilized recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |