Active Recombinant Human CSF2 Protein

• Cat.No.: CSF2-142H
• Product Overview: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our Yeast expression system and the target gene encoding Ala18-Glu144 is expressed.

Specification

• Description: GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages, and eosinophils, GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
• Source: Pichia pastoris
• Species: Human
• Form: Lyophilized from a 0.2 um filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5.
• Bio-activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/ml.
• Molecular Mass: Predicted Molecular Weight: 14.4kDa
  Apparent Molecular Weight: 24-35kDa, reducing conditions.
• AA Sequence: Ala18-Glu144
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Quality Statement: Purity Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 [ Homo sapiens (human) ]
• Official Symbol: CSF2
• Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; GMCSF
• Gene ID: 1437
• mRNA Refseq: NM_000758.4
• Protein Refseq: NP_000749.2
• MIM: 138960
• UniProt ID: P04141