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Active Recombinant Human CUL1/RBX1/SKP1

Cat.No. : CUL1-147H
Product Overview : Recombinant human CUL1 (amino acid residues 1-776), RBX1 (amino acid residues 1-108), SKP1 (amino acid residues 1-160) was expressed in Insect (Sf21) cells.
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Description : Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003).
Source : Insect cells
Species : Human
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : E3 Ligase Assay: The activity of Cul1/Rbx1/Skp1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase GST-DCNL1 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of GST-DCNL1 at 4?C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.
Molecular Mass : Cul1: ~89.9 kDa; Rbx1: ~12.3 kDa; Skp1: ~18.3
Purity : >95% by InstantBlue™ SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Publication :
Lys29-linkage of ASK1 by Skp1? Cullin 1? Fbxo21 ubiquitin ligase complex is required for antiviral innate response." (2016)
Gene Name : CUL1 cullin 1 [ Homo sapiens ]
Official Symbol : CUL1
Synonyms : CUL1; cullin 1; cullin-1; CUL-1; MGC149834; MGC149835;
Gene ID : 8454
mRNA Refseq : NM_003592
Protein Refseq : NP_003583
MIM : 603134
UniProt ID : Q13616
Chromosome Location : 7q36.1
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Cell Cycle, organism-specific biosystem;
Function : protein binding; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What techniques are used to study the expression and function of CUL1 protein in clinical research? 02/09/2023

Techniques such as immunohistochemistry, Western blotting, and gene expression analysis are commonly used to study CUL1 protein expression and function in clinical research.

Is CUL1 protein involved in any other diseases besides cancer? 11/11/2022

Yes, CUL1 protein has been implicated in various diseases, including neurodegenerative disorders and cardiovascular diseases, due to its role in protein degradation and cellular regulation.

Can CUL1 protein be used as a biomarker for cancer diagnosis? 05/07/2020

While CUL1 protein levels are altered in certain cancers, it is not commonly used as a standalone biomarker. However, it may be part of a panel of markers used for cancer diagnosis.

Are there any genetic disorders associated with CUL1 protein mutations? 08/21/2017

Yes, mutations in the CUL1 gene have been linked to a rare genetic disorder called Cullin-Ring Ligase 1 Syndrome, which is characterized by developmental delays and intellectual disabilities.

How does the dysregulation of CUL1 protein impact cell cycle progression? 02/18/2017

Dysregulation of CUL1 protein can lead to the abnormal degradation of cell cycle regulators, resulting in uncontrolled cell division and potentially contributing to tumorigenesis.

Customer Reviews (3)

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06/16/2021

    I highly recommend the CUL1 protein for a variety of research applications.

    05/08/2016

      It demonstrates excellent performance in ELISA, consistently delivering accurate and reliable results.

      04/05/2016

        the CUL1 protein has proven to be an invaluable tool in protein electron microscopy structure analysis.

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