Recombinant Full Length Human CUL1 Protein, C-Flag-tagged

• Cat.No.: CUL1-1937HFL
• Product Overview: Recombinant Full Length Human CUL1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 89.5 kDa
• AA Sequence: MSSTRSQNPHGLKQIGLDQIWDDLR AGIQQVYTRQSMAKSRYMELYTHVY NYCTSVHQSNQARGAGVPPS KSKKGQTPGGAQFVGLELYKRLKEF LKNYLTNLLKDGEDLMDESVLKFYT QQWEDYRFSSKVLNGICAYL NRHWVRRECDEGRKGIYEIYSLALV TWRDCLFRPLNKQVTNAVLKLIEKE RNGETINTRLISGVVQSYVE LGLNEDDAFAKGPTLTVYKESFESQ FLADTERFYTRESTEFLQQNPVTEY MKKAEARLLEEQRRVQVYLH ESTQDELARKCEQVLIEKHLEIFHT EFQNLLDADKNEDLGRMYNLVSRIQ DGLGELKKLLETHIHNQGLA AIEKCGEAALNDPKMYVQTVLDVHK KYNALVMSAFNNDAGFVAALDKACG RFINNNAVTKMAQSSSKSPE LLARYCDSLLKKSSKNPEEAELEDT LNQVMVVFKYIEDKDVFQKFYAKML AKRLVHQNSASDDAEASMIS KLKQACGFEYTSKLQRMFQDIGVSK DLNEQFKKHLTNSEPLDLDFSIQVL SSGSWPFQQSCTFALPSELE RSYQRFTAFYASRHSGRKLTWLYQL SKGELVTNCFKNRYTLQASTFQMAI LLQYNTEDAYTVQQLTDSTQ IKMDILAQVLQILLKSKLLVLEDEN ANVDEVELKPDTLIKLYLGYKNKKL RVNINVPMKTEQKQEQETTH KNIEEDRKLLIQAAIVRIMKMRKVL KHQQLLGEVLTQLSSRFKPRVPVIK KCIDILIEKEYLERVDGEKD TYSYLA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway

Gene Information

• Gene Name: CUL1 cullin 1 [ Homo sapiens (human) ]
• Official Symbol: CUL1
• Synonyms: MGC149834; MGC149835
• Gene ID: 8454
• mRNA Refseq: NM_003592.3
• Protein Refseq: NP_003583.2
• MIM: 603134
• UniProt ID: Q13616