Active Recombinant Human GSTP1 Protein (1-210aa), N-His tagged

• Cat.No.: GSTP1-22H
• Product Overview: Recombinant human GSTP1 protein (1-210aa) was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Liquid
• Bio-activity: > 80 unit/mg, and is defined as the amount of enzyme that conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 centigrade.
• Molecular Mass: 27.4 kDa (246aa) confirmed by MALDI-TOF
• Protein Length: 1-210aa
• AA Sequence: MPPYTVVYFPVRGRCAALRMLLADQ GQSWKEEVVTVETWQEGSLKASCLY GQLPKFQDGDLTLYQSNTILRHLGR TLGLYGKDQQEAALVDMVNDGVEDL RCKYISLIYTNYEAGKDDYVKALPG QLKPFETLLSQNQGGKTFIVGDQIS FADYNLLDLLLIHEVLAPGCLDAFP LLSAYVGRLSARPKLKAFLASPEYV NLPINGNGKQ
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.

Gene Information

• Gene Name: GSTP1 glutathione S-transferase pi 1 [ Homo sapiens (human) ]
• Official Symbol: GSTP1
• Synonyms: GSTP1; glutathione S-transferase pi 1; FAEES3, GST3; glutathione S-transferase P; GSTP; GSTP1-1; GST class-pi; deafness, X-linked 7; fatty acid ethyl ester synthase III; PI; DFN7; GST3; FAEES3
• Gene ID: 2950
• mRNA Refseq: NM_000852
• Protein Refseq: NP_000843
• MIM: 134660
• UniProt ID: P09211