Active Recombinant Human HDAC8 Protein

• Cat.No.: HDAC8-314H
• Product Overview: Recombinant Human HDAC8 Protein is produced by HEK293 cells expression system.

Specification

• Description: Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA).
• Source: HEK293 cells
• Species: Human
• Form: Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.5.
• Bio-activity: Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis
• Molecular Mass: 41 kDa
• Protein length: amino acids 1–377
• Purity: ≥95% by SDS-PAGE
• Notes: For Research Use Only! Not For Use in Humans.
• Storage: Store at -80 centigrade.
• Handling: Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: HDAC8 histone deacetylase 8 [ Homo sapiens ]
• Official Symbol: HDAC8
• Synonyms: HDAC8; histone deacetylase 8; HDACL1, histone deacetylase like 1; RPD3; histone deacetylase-like 1; HD8; CDA07; HDACL1
• Gene ID: 55869
• mRNA Refseq: NM_001166418
• Protein Refseq: NP_001159890
• MIM: 300269
• UniProt ID: Q9BY41