| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
amino acids 1–377 |
| Description : |
Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA). |
| Form : |
Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.5. |
| Bio-activity : |
Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis |
| Molecular Mass : |
41 kDa |
| Purity : |
≥95% by SDS-PAGE |
| Notes : |
For Research Use Only! Not For Use in Humans. |
| Storage : |
Store at -80 centigrade. |
| Handling : |
Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles. |
